ngs ONCO anti-EGFR Diagnostic Kit

This CE-IVD diagnostic kit is intended for target amplification of hot-spot areas in selected oncogenes relevant for target therapy of oncological patients (colorectal cancer, non-small cell lung cancer). It is designed to prepare a library of samples for sequencing on the Illumina MiSeq® platform. The kit includes free use of the very user-friendly ERMI evaluation software.

Cat. no. 3301-024 ngsONCO anti-EGFR 24 reactions
Cat. no. 3301-064 ngsONCO anti-EGFR 64 reactions

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Clinical implications

Diagnostic kit ngs ONCO anti-EGFR is a CE-IVD certified test that consists of a clinically relevant gene panel for target-therapy optimization of oncologic patients. It helps to determine suitability of deployed monoclonal antibodies Cetuximab (Erbitux) and Panitumumab (Vectibix) in colorectal cancer (CRC) that target EGFR (Epidermal growth factor receptor) protein by confirming/excluding mutations in KRAS, NRAS, and BRAF genes, because patients with these mutations do not profit from anti-EGFR treatment.

Parameters of the diagnostic kit

  • Diagnostic kits for next-generation sequencing on Illumina MiSeq® platform
  • Up to 64 samples per sequencing run (when used with v2 300 cycles chemistry)
  • Panel of 8 relevant genes for molecular diagnostics of somatic mutations in colorectal cancer, lung cancer, and melanomas using the anti-EGFR antibody therapy
  • Carefully designed primers for single-tube amplicon multiplex PCR library preparation providing uniform coverage
  • Suitable with the FFPE samples and input DNA in amounts as low as 20 ng
  • Straightforward library preparation protocol with short hands-on time
  • Developed in collaboration with experts from the Clinic of Oncology, General University Hospital in Prague
  • Bioinformatic software solution provides user friendly data analysis and generates a report

Kit Composition

Compound Purpose
Master Mix 1
Master Mix 2
Primer Mix anti-EGFR Multiplex PCR promer mix
Primer F01-F08 Sample specific barcode sequence
Primer R01-R08 Sample specific barcode sequence
SP_Read1 Sequencing primer
SP_IndexR1 Sequencing primer
SP_Read2 Sequencing primer
Magnetic beads Library purification step
Elution buffer Library purification step

Panel of genes included in ngs ONCO anti-EGFR  diagnostic kit:

KRAS chr 12 exon 2 exon 3 exon 4
BRAF chr 7 exon 11 exon 15
NRAS chr 1 exon 2 exon 3 exon 4
EGFR chr 7 exon 18 exon 19 exon 20 exon 21
PIK3CA chr 3 exon 21
AKT1 chr 14 exon 3
HER2 chr 17 exon 25
SMAD chr 18 exon 12

This product was developed with support of Technological Agency of the Czech Republic (project TA04010838)